HPLC Analysis & Peptide COA Verification | BioLabs Research
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Methods

Peptide COA Verification: HPLC Analysis and MS Identity

CAS and sequence on the catalog page are public identity. Peak area, observed mass, and net peptide content are lot data. They sit on the COA that matches the vial.

  • HPLC Area percent at a stated wavelength
  • Mass spec Observed mass versus theoretical mass
  • COA Lot, laboratory, method, chromatogram
Research-use compounds. Laboratory context only.

Overview

HPLC analysis of peptides: what area percent reports

HPLC reports area percent. Main-peak area divided by total detected peak area at a stated wavelength. A chromatogram ratio, not the milligrams in the vial.

Mass spectrometry: confirming the named sequence

Mass spectrometry confirms identity. Observed mass is checked against the theoretical mass of the named sequence. Area percent does not name the molecule.

How to verify a peptide COA in five fields

The COA is lot-specific. Lot number, date, laboratory, method, and a chromatogram or peak table travel with that vial.

Lot number matches the vial

Lot number travels with that vial.

Method and wavelength are stated

Method, and area percent at a stated wavelength, travel with that vial.

Chromatogram or peak table is attached

A chromatogram or peak table travels with that vial.

Observed vs theoretical mass

Observed mass is checked against the theoretical mass of the named sequence.

Laboratory name and test date

Date and laboratory travel with that vial.

HPLC purity vs net peptide content

HPLC percent is not net peptide content. TFA, acetate, and water are often invisible at 214 nm. Net peptide is a separate assay.

Research use only. In-vitro laboratory reagents. Not medicines, not supplements, not for human use.

Catalog listings with lot COAs

Lab COA PDFs are published per product where available in our COA Library (others on request) · How to read a peptide COA

06 · Catalog

Listed prices

USD list prices for the SKUs below. The bar length is price, not purity.

CATALOG PRICE · USD AOD-9604 $85 BPC-157 $88 NAD+ $99 Tesamorelin / Ipamorelin $119 BPC-157 / TB-500 Blend $125

AOD-9604 $85 · BPC-157 $88 · NAD+ $99 · Tesamorelin / Ipamorelin $119 · BPC-157 / TB-500 Blend $125. Catalog prices, not a quality ranking.

Request a lot COA Request a Quote

Need several lots documented? Written quote within one business day.

07 · FAQ

Questions

Use and limits

What is RUO?

Research Use Only. These compounds are sold for in-vitro and laboratory research. They are not approved medicines, not supplements, and not for human consumption, self-administration, or therapeutic use.

Do you dose?

No. No body-weight charts, injection instructions, or treatment advice.

Documents

How do I verify a peptide COA?

Match the lot on the certificate to the vial label, then check the stated HPLC method and wavelength, the chromatogram, the MS observed vs theoretical mass, and the laboratory name and date.

Why does HPLC analysis need MS as well?

HPLC area percent reports how UV-detected peak area splits. It does not name the molecule. MS confirms that the main species matches the expected mass.

Can I get the COA for a specific lot?

Yes. Request it by email from the product page with the lot number from the vial.

Why two tests?

HPLC reports how UV-active peak area splits. Mass spectrometry checks whether the species matches the expected mass. A complete lot record uses both.

Why is there no purity percent on the homepage?

A percentage without lot, method, and wavelength is not a result. Those figures are issued on the COA for that lot.

What is a lot?

A manufacturing batch. The lot on the vial should match the lot on the certificate. Different lots of the same SKU are different records.

Fulfilment

How should research peptides be stored?

Storage requirements can vary between compounds and formulations. Researchers should follow the storage conditions supplied with the specific product and its documentation rather than applying one storage method to every peptide.

References

Sources

Method literature and public registries. Lot HPLC, MS, and net peptide figures remain on the matching COA.

  1. D’Hondt M, et al. Related impurities in peptide medicines. J Pharm Biomed Anal. 2014;101:2–30. https://doi.org/10.1016/j.jpba.2014.06.012
  2. Verbeke F, et al. Quality evaluation of synthetic quorum sensing peptides used in R&D. J Pharm Anal. 2015;5(3):169–181. https://doi.org/10.1016/j.jpha.2014.12.002
  3. ICH Q3C. Impurities: Guideline for Residual Solvents. https://www.ich.org/page/quality-guidelines
  4. IUPAC Gold Book, L03578 — high-performance liquid chromatography. https://goldbook.iupac.org/terms/view/L03578
  5. CAS 137525-51-0, BPC-157 sequence GEPPPGKPADDAGLV (literature identity). Lot identity remains the observed mass on the COA.
  6. PubChem CID 71300630 / CAS 221231-10-3, AOD-9604. https://pubchem.ncbi.nlm.nih.gov/compound/71300630
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